The Effects of Salinity and Methyl Jasmonate on Morphological and Biochemical Characteristics and Photosynthetic Pigments Content in Two Basil Cultivars

Authors

Abstract

To evaluate the interaction effects of salinity and methyl jasmonate on some morphological and biochemical characteristics of two cultivars of basil, a pot experemint was carried out using factors of salinity (0, 30, 60 and 90 mM) and spraying methyl jasmonate (0, 5.0 mM) in two commercial cultivars (Rubi and Genove). The results showed that salt treatments had a significant effect on all traits, except the content of carotenoids. With increasing salinity levels up to 90 mM plant height, shoot dry weight, total carbohydrates and relative water content in both cultivars decreased, whereas the weight of the root, electrolyte leakage, proline, chlorophyll a and total chlorophyll increased. Methyl jasmonate treatment had a significant effect at 1% level of probability on stem fresh weight, total carbohydrates, prolin and total chlorophyll, and at 5% level of probability on plant height, root fresh and dry weight and chlorophyll a and b, but it was ineffective on other characteristics. The interaction effect of salinity × methyl jasmonate was significant on chlorophyll a and b and total chlorophyll (P≥ 0.01) and interaction effect of salinity × methyl jasmonate × cultivar was significant on root fresh weight, total carbohydrates, chlorophyll a and total chlorophyll (P≥ 0.01), but it was ineffective on other traits. The overall results of this study showed that cultivars of basil had different response to salt stress and use of methyl jasmonate. The application of methyl jasmonate in stress condition improved charactrestics of morphological, biochemical and photosynthetic pigments in the basil.

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Abraham, E. M., Huang, B., Bonos, S. A., and Meyer, W. A. 2004. Evaluation of drought resistance for Texas bluegrass, Kentucky bluegrass, and their hybrids. Crop Science 44: 1746-1753. Archangi, A., and Khodambashi, M. 2015. Effects of salinity stress on morphological characteristics, essential oil content and ion accumulation in basil (Ocimum basilicum) plant under hydroponic conditions. Science and Technology of Greenhouse Culture 17(5): 125-138 (in Persion). Azarnivand, H., and Qorbani, M. 2007. The effect of sodium chloride on the germination of two species of pasture Puccinella distans L. and Frankenia hirsuta L. Iranian Journal of Range and Desert Reseach 4(3): 352-358 (in Persian). Babst, B. A., Ferrieri, R. A., Gray, D. W., Lerdau, M., Schlyer, D. J., Schueller, M.,Thrope, M. R., and Orians, C. M. 2005. Jasmonic acid induces rapid changes in carbon transport and partitioning in Populus. New Phytologist 167: 63-72. Bari, R., and Jones, J. D. G. 2009. Role of plant hormones in plant defense responses. Plant Molecular Biology 69:473–488. Çiçek, N., and Çakirlar, H. 2002. The effect of salinity on some physiological parameters in two maize cultivars. Bulgarian Journal of Plant Physiology 28(1–2): 66-74. Comparot, S. M., Graham, C. M., and Reid, D. M. 2002. Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots. Journal of Plant Growth Regulation 38: 21-30. Creelman R. A., and Mullet, J. E. 1995. Jasmonic acid distribution and action in plants: Regulation during development and response to biotic and abiotic stress. Proceedings of the National Academy of Sciences, USA 92: 4114-4119. Dere, S., Gunes, T., and Sivaci, R. 1998. Spectrophotometric determination of chlorophyll- a, b and total carotenoids contents of some algae species using different solvents. Botany 22: 13-17. Eshqizade Samani, H., Kafi, M., Nezami, A., and Khoshgoftar Manesh, A. H. 2013. The effect of salinity on leaf water status, proline, total soluble sugars and antioxidant activity of blue panic grass. Science and Technology of Greenhouse Culture 18(5): 11-24 (in Persian). Etesami, M., and Galeshi, A. 2008. Assess the reaction of ten genotypes to salinity during seed germination and seedling growth. Journal of Agricultural Sciences and Natural Resources 15(5): 39-46 (in Persian). Fedina, I. S., and Benderliev, K. M. 2000. Response of Secendesmusincras satulus to salt stress as affected by methyl jasmonate. Biologia Plantarum 43(4): 625-627. Garratt, L. C., Janagoudr, B. S., Lowe, K. C., Anthony, P., Power, J. B.و and Davey, M. R. 2002. Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine 33(4): 502-511. Han, H. S., and Lee, K. D. 2005. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Research Journal of Agriculture and Biological Sciences 1(3): 210-215. Hedge, J. E., and Hofreiter, B. T. 1962. Carbohydrate Chemistry. Academic Press, New York, USA. Javanmardi, J., Khalighi, A., Kashi, A., Bais, H. P., and Vivanco, J. M. 2002. Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. Journal of Agricultural and Food Chemistry 50(21): 5878-5883. Kazemzadeh Haqiqi, A. 2008. Evaluation of salinity tolerance in relation to proline and soluble sugars accumulation on 9 forage sorghum cultivars. Plant Science Research 1(1): 15-23 (in Persian). Keramt, B., and Daneshmand, F. 2012. Dual role of methyl jasmonate in physiological responses of soybean (Glycine max L.) plant. Journal of Plant Process and Function 1(1): 26-38 (in Persian). Li, L., van Staden, J., and Jager, A. K. 1998. Effect of plant growth regulators on the antioxidant system in seedling of two maize cultivars subjected to water stress. Plant Growth Regulation 25: 81-87. Maciejewska, B., and Kopcewicz, J. 2002. Inhibitory effect of methyl jasmonate on flowering and elongation growth in Pharbitis nil. Plant Growth Regulation 21: 216-223. Marcum, K. B. 1998. Cell membrane theromotability and whole-plant heat tolerance of Kentucky bluegrass. Crop Science 38: 1214-1218. Munnes, R. 2002. Comprative physiology of salt and water stress. Plant Cell Environment 25(2): 239-250. Nazarbigi, A., and Baluchi, N. 2011. Evalute the interaction effects of salicylic acid and sodium chloride on the activity of catalase and peroxidase on two canola varieties. Physiological Crops 3(3): 196-204 (in Persian). Norafkan, H., Angoti, F., and Hasani Dastgiri, S. 2012. Effects of salicylic acid on chlorophyll and some morphological properties of basil (Ocimum basilicum) under sodium chlorid stress. The First National Conference on Ways to Achieve Sustainable Development, Gorgan, Iran (in Persian). Pandey, V. K., and Saxena, H. K. 1987. Effects of soil salinity on chlorophyll, photosynthesis, respiration and ionic composition at various growth stages in paddy. Indian Journal of Agricultural Chemistry 20(2): 40-155. Piotrowska, A., Bajguz, A., Godlewska Zylkiewicz, B., and Czerpak, R. 2009. Jasmonic acid modulator of lead toxicity in aquatic plant Wolffia arrhiza (Lemnaceae). Environmental and Experimental Botany 66: 507-513. Popova, L., Ananieva, E., Hristova, V., Christov, K., Georgieva, K., Alexieva, V., and Stoinova, Z. H. 2003. Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology 18: 133-152. Qaseminezhad, P., Mehrafarin, A., Biglori, M., and Mansuri, M. 2011. The effect of light and salinity stress on germination and growth seedlings of artichokes. Proceedings of the Second National Conference on Seed Science and Technology, Islamic Azad University of Mashhad, Mashhad, Iran (in Persian). Ritchie, S.W., Nguyen, H.T., and Haloday, A.S. 1990. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science 30: 105-111. Rossato, L., Le Dantec, C., Laine, P., and Ourry, A. 2002. Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: identification, and immunolocalization of a putative taproot storage glycoprotein. Journal of Experimental Botany 53: 265-275. Sai Kachout, S., Ben Mansoura, A., Jaffel Hamza, K., Leclerc, J. C., Rejeb, M. N., and Ouerghi, Z. 2011. Leaf–water relations and ion concentrations of the halophyte Atriplex hortensis in response to salinity and water stress. Acta Physiologiae Plantarum 33(2): 335-342. Salimi, F., Shekari, F., Azimi, M. R., and Zangani, A. 2010. Role of methyl jasmonate on improving salt resistance through some physiological characters in German chamomile, (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants 27(4): 700-711 (in Persian). Shafizade, M., Parsa, S., Ahmadi, K., and Alizadeh, R. 2011. Effect of seed preparation on germination improving and seedling growth of medicinal plant artichokes under salt stress. Proceedings of the Second National Conference on Seed Science and Technology, Islamic Azad University of Mashhad, Mashhad, Iran. pp. 213-217 (in Persian). Shahbazi, M., and Mohaqeqdust, Z. 1996. Evalute of sodium chloride effects on growth and accumulation of organic and mineral compounds in wheat. Iranian Journal of Agricultural Sciences 27(4): 70-78 (in Persian). Shirani Bidabadi, S., Mehri, H., Ghobadi, C., Baninasab, B., and Afazel, M. 2013. Morphological, physiological and antioxidant responses of some Iranian grapevine cultivars to methyl jasmonate application. Journal of Crop Science and Biotechnology 16(4): 277- 283. Srivastava, L. M. 2002. Plant Growth and Development. Hormones and Environment. Academic Press, New York, USA. Teimouri, A., and Jafari, M. 2010. The effects of salinity stress on some of anatomical and morphological characteristics in three Salsola species: S. rigida, S. dendroides, S. richteri. Iranian Journal of Range and Desert Reseach 17(1): 21-34 (in Persian). Ueda, J., and Saniewski, M. 2006. Methyl jasmonate–induced stimulation of chlorophyll formation in the basal part of tulip bulbs kept under natural light conditions. Journal of Fruit and Ornamental Plant Reserch 14: 199-210. Wang, S. Y. 1999. Methyl Jasmonate reduces water stress in strawberry. Journal of Plant Growth Regulation 18: 127-134. Weidhase, R., Kramell, H. M., Lehmann, J., Liebisch, H. W., Lerbs, W., and Parthier, B. 1987. Methyl jasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Science 51: 177-186. Yang, F., Xiao, X., Zhang, S., Korpelainen, H., and Li, C. 2009. Salt stress responses in Populus cathayana Rehder. Plant Science 176: 669-677. Yarnia, M. 2007. Evaluation of some physiological indices of forage sorghum cultivars under salt stress conditions. Journal of Agricultural Sciences, Islamic Azad University of Tabriz 1(1): 1-15 (in Persian). Zhao, G. Q., Ma, B. L., and Ren, C. Z. 2007. Growth, gas exchange, chlorophyll fluorescence and ion content of Naked oat in response to salinity. Crop Science 47: 123-131.