Effect of Freezing Temperatures on Different Seedling Ages of Chickpea Genotypes under Controlled Conditions

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Abstract

In order to study the effects of sub-zero temperatures on advanced chickpea genotypes, this study was carried out as split-split plot based on randomized complete block design with three replications under controlled conditions (cold room) at Maragheh Dryland Agricultural Research Station during 2013-2014 cropping season. Different seedling stages (1,3 and 6 weeks after planting) were in main plots, different sub-zero temperatures (0, -5, -10, -15 and -20 °C) in sub plots and chickpea genotypes (ILC3279, FLIP00-75, ILC482, FLIP00-84, Sel.96th.11439, ILC 533 and cultivar Saral) in sub-sub plots. Results showed that seedling age did not significantly affect the frost resistance ratio (FRR), but the highest and the lowest FRR were observed in seedlings with 3 and 6 weeks age, respectively. Cold severity had significant effect (p≤ 0.01) on FRR and with increasing cold severity from 0 to -20°C, FRR of genotypes was decreased. Differences among chickpea genotypes were significant (p≤ 0.01) and Saral cultivar with FRR = 0.89 showed an acceptable level of cold tolerance comparing with the other genotypes.

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