Optimization of in vitro Culture for Gerbera cv. Tropic Blend

Immature capitulum of gerbera cultivar of Tropic Blend, after disinfection and removal of sepals, divided into four parts, and cultured on 1/2MS medium supplemented with 0.05, 0.1, 0.5 and 1 mg/l TDZ and 0.1 mg/l IAA. The remainings were also cultured in 1/2MS medium, supplemented with 5, 10 and 15 mg/l BAP and 1 mg/l IAA. Cultures were incubated in dark conditions and then transferred to the light. There was not any prolifration in 1/2 MS medium suplemented with different concentrations of BAP. The highest number of plantlets was obtained in 1/2 MS supplemented with 0.05 mg/l TDZ. For prolifration, plantlets were produced in MS medium supplemented with 2, 4, 6 and 8 mg/l kinetin. The highest and lowest number of plantlets were producted in MS medium with 2 and 8mg/l kinetin and 1 mgl IAA, respectively. The highest number of leaves was produced in 2 and 4 mg/l kinetin. For rooting, plantlets were cultured in MS medium supplemeted with 0.5 and 1 mg/l NAA. The highest number of roots was produced in MS with 0.5 mg/l NAA. For acclimatization the rooted plantlets were transferred into pots. They were throughly acclimized.