Study of Biological Control of Root-Knot Nematode, Meloidogyne javanica (Trube) Chitwood, in Tomato by Trichoderma harzianum Rifai in Greenhouse and Quantitative Changes of Phenolic Compounds in Plant

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Abstract

The biological control agent, Trichoderma harzianum BI, was evaluated against the root-knot nematode Meloidogyne javanica in tomato.Tomato roots were inoculated by different concentration of Trichoderma harzianum BI using root-dip method. The pots were immersed in aluminum trays containing water 27 ° Ċ and transfered to a greenhouse. In greenhouse the concentration of 106 spore/ml of Trichoderma was effective for control of nematode root galls, root fresh weight, egg mass and the number of egg per mass reduced and shoot fresh weight increased. The number of root galls in treatment of 106 spore/ml was significantly greater than those of 103,104,105 spore/ml and control (without antagonist), but there were no significant differences among 106,107and108 spore/ml (p≤0.05). Results of quantitive changes in phenolic compounds in tomato cv. King Stone showed that, there were significant differences between Trichoderma inoculated plants and control.Total phenol content in plant inoculated with fungus and nematode was greater than those plants inoculated only with Trichoderma. The maximum phenol content was observed eight days after inoculation. According to these results T. harzianum can induce plant defence mechanisms (especially phenolic compounds) and reduces nematode disease rate.

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Seed and Plant Production Journal
Volume 25, Issue 3
September 2009
Pages 259-272

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